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多聚半乳糖醛酸酶(PG)测试盒

多聚半乳糖醛酸酶(PG)测试盒

货号:PG-2-V
方法学: 可见分光光度法

规格:50管/24样
价格:询价
Price:Inquiry






540.00
540.00
  
商品描述

货号:PG-2-V
方法学: 可见分光光度法

规格:50管/24样
价格:询价
Price:Inquiry







测定意义: 多聚半乳糖醛酸酶(EC3.2.1.15)是一种细胞壁结合蛋白,可以催化果胶分子中 α-(1,4)-聚半乳糖醛酸的裂 解,参与果胶的降解,使细胞壁结构解体,导致果实软化,与果实成熟、叶和花的脱落、病原物防御,细 胞伸展发育以及木质化有关,在植物抗病性和食品贮藏保鲜领域具有较高的研究价值。 

测定原理: 多聚半乳糖醛酸酶水解果胶酸生成半乳糖醛酸,具有还原性醛基,与 DNS 试剂反应生成红棕色物质,在 540nm 有特征吸收峰,测定 540nm 处吸光值变化可计算得多聚半乳糖醛酸酶活性。

测定意义:

多聚半乳糖醛酸酶(EC3.2.1.15)是一种细胞壁结合蛋白,可以催化果胶分子中α-(1,4)-聚半乳糖醛酸的裂解,参与果胶的降解,使细胞壁结构解体,导致果实软化,与果实成熟、叶和花的脱落、病原物防御,细胞伸展发育以及木质化有关,在植物抗病性和食品贮藏保鲜领域具有较高的研究价值。

测定原理:

多聚半乳糖醛酸酶水解果胶酸生成半乳糖醛酸,具有还原性醛基,与DNS试剂反应生成红棕色物质,在540nm有特征吸收峰,测定540nm处吸光值变化可计算得多聚半乳糖醛酸酶活性。


Q:多聚半乳糖醛酸酶(PG)活性(可见分光光度法 24S),PG-F24-N(1620)可以用用紫外分光光度计的吧?波长为190~1100nm的紫外分光光度计能测吗?标准样品浓度是指什么?意思是我只要测出对照组和样品组的吸光度然后加上0.008然后除以组织质量乘以2.523就可以得出活性了,对吗?

A:这个方法就是分光法的,用紫外分光光度计可以的,检测设置波长540nm。这是我们实验室做试剂盒时做的不同浓度半乳糖醛酸的标准曲线,客户可以自备标准品做标准曲线。如果不再单独做标准曲线的话,可以根据我们说明书提供的标准曲线和计算方式即可,一般误差5%~10%。


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